human primary dermal fibroblasts hdfa cells  (ATCC)

Journal: Bioresources and Bioprocessing

Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential

doi: 10.1186/s40643-025-00986-2

Figure Lengend Snippet: Viability of human dermal fibroblasts (HDFa), breast adenocarcinoma cells (MCF-7), and colorectal adenocarcinoma cells (Caco-2) after 48 h exposure to arenin. Data are expressed as the percentage of MTS-formazan absorbance (490 nm) relative to untreated controls (mean ± SD, n = 3). Statistical analysis was performed with one-way ANOVA followed by Tukey’s multiple-comparison test; Different letters abcde indicate statistical differences analyzed using the Tukey test ( p < 0.05)

Article Snippet: Human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (Caco-2), and human primary dermal fibroblasts (HDFa) cells were purchased from the American Type Culture Collection (ATCC®, Manassas, VA, USA).

Techniques: Comparison

Journal: Bioresources and Bioprocessing

Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential

doi: 10.1186/s40643-025-00986-2

Figure Lengend Snippet: Effect of arenin on HDFa cell migration under high glucose (HG) and serum-deprived (FBS–) conditions. Scratch wound healing assays were performed in monolayers treated with arenin (31.25–1000 µg mL −1 ), Centella asiatica extract (CA, 10 µg mL −1 ), or left untreated (C–). A , C Time course of wound retraction (%) under FBS– and HG conditions at 24, 48, and 72 h. B , D Wound closure (%) after 72 h in FBS– and HG conditions. E Phase contrast micrographs at 0 and 72 h under FBS– (upper) and HG (lower) conditions show C–, CA, and arenin treatments. Dashed lines separate controls from arenin. Complete closure is observed in arenin- and CA-treated cultures, while sizeable gaps persist in C–. Bars/data points = mean ± SD ( n = 3). One-way ANOVA with Tukey’s post hoc test; groups sharing the same lowercase letter are not significantly different ( p > 0.05). Scale bars = 200 µm

Article Snippet: Human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (Caco-2), and human primary dermal fibroblasts (HDFa) cells were purchased from the American Type Culture Collection (ATCC®, Manassas, VA, USA).

Techniques: Migration

Journal: Cell Death & Disease

Article Title: Identification of a small molecule targeting EPLIN as a novel strategy for the treatment of pediatric neuroblastoma and medulloblastoma

doi: 10.1038/s41419-025-07876-7

Figure Lengend Snippet: A Workflow of the cell-based drug screening. A total of 1581 compounds were assessed in a cell-based screening in 96-well plates in both 2D- and 3D-conditions to find compounds that exhibited pan toxicity to neuroblastoma cells. Five compounds, FLIX1 (NSC105827), FLIX2 (NSC607097), FLIX3 (NSC354844), FLIX4 (NSC330770), and FLIX5 (NSC328403), were identified showing >50% cytotoxicity to 5 different neuroblastoma (NB) spheroids with or without MYCN overexpression at 1 µM after 72 h treatment. B Dose-response of the 5 identified compounds, FLIX1– FLIX5, which show a greater toxicity on 6 neuroblastoma cell lines compared to immortalized hTERT RPE-1 cells in 2D-condition. One representative experiment with 3 technical replicates is shown (mean ± SD). C Dose-response of FLIX4 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). D Dose-response of FLIX5 on human medulloblastoma cells and mouse derived medulloblastoma cells. One representative experiment with 3 technical replicates is shown (mean ± SD). HDF: human dermal fibroblast (normal control cell line). DAOY human medulloblastoma cells, D283 human medulloblastoma cells, high express MYC. GMYC1: mouse medulloblastoma cells, high express MYC . GTML-S1: mouse medulloblastoma cells, high express MYCN ; GTML2: mouse medulloblastoma cells, high express MYCN ; GTML3: mouse medulloblastoma cells, high express MYCN . E Dose-response of FLIX5 on neuroblastoma and immortalized hTERT RPE-1 spheroids. One representative experiment with 3 technical replicates is shown (mean ± SD). F Colony formation assay of SK-N-AS spheroids treated with FLIX5 at concentrations of 43 nM, 129 nM, and 388 nM for 72 h. After exposure, the spheroids were dissociated, and the cells were cultured until cells reached confluency under control conditions. G Quantification of the colony formation assay in ( F ) revealed statistically significant differences between the control and FLIX5 treatments at concentrations of 129 nM and 388 nM. One representative experiment with 5 technical replicates is shown (mean ± SD; t test, p < 0.05 is considered significant).

Article Snippet: hTERT-immortalized retinal pigment epithelial cell line (Cat. #CRL-4000) and neuroblastoma cell lines SK-N-AS (Cat. #CRL-2137), SH-SY5Y (Cat. #CRL-2266), SK-N-SH, (Cat. #HTB-11), CHP-212 (Cat. #CRL-2137), IMR-32 (Cat. #CRL-127), SK-N-BE2 (Cat. #CRL-2271) and medulloblastoma cell lines HDFa (Cat. #PCS-201-012), D283 (Cat. #HTB-185) and Daoy (Cat. #HTB-186) was obtained from (ATCC, Manassas, VA, USA). hTERT, SK-N-AS, SH-SY5Y, SK-N-SH, CHP-212, IMR-32, SK-N-BE [ ] was maintained in 1:1 ratio of EMEM (ATCC, Cat. #30-2003) and Ham’s F-12 Nutrient Mix (Thermo Fisher Scientific, Waltham, MA, USA, Cat. #11765054) supplemented with 1% penicillin streptomycin (Thermo Fisher Scientific, Cat. #15140-122) and 10% FBS (Thermo Fisher Scientific, Cat. #10270-106).

Techniques: Drug discovery, Over Expression, Derivative Assay, Control, Colony Assay, Cell Culture